The distribution and composition of C-heterochromatin in four species of Mictini (Coreinae), viz., Anoplocnemis compressa (Dallas, 1852), Anoplocnemis binotata (Distant, 1918), Ochrochira nigrorufa (Distant, 1889) and Prionolomia sp., have been analyzed by C-banding and DAPI/CMA₃ sequence specific staining. Cytogenetically, the possession of holokinetic chromosomes and a pre-reductional type of meiosis for sex chromosomes characterize these four species. The C-banding pattern has been found to be species-specific. In A. compressa, C-bands are thick and terminal, whereas in A. binotata, very thin C-bands are seen interspersed throughout the length of chromosomes. In Ochrochira nigrorufa, thick C-bands are present at terminal and interstitial regions. In Prionolomia sp., two conspicuous terminal C-bands are observed only on the largest autosomal pair while the rest of the complement is completely C-negative. This unique pattern can serve as a powerful cytological marker. Constitutive heterochromatin has been found to be rich in both AT and GC base pairs in all the studied species.