Internal Medicine
Online ISSN : 1349-7235
Print ISSN : 0918-2918
ISSN-L : 0918-2918
Successful Diagnosis Using Scab for PCR Specimen in Tsutsugamushi Disease
Akihiro ONO, Kumiko NAKAMURA, Seiichi HIGUCHI, Yoshihiro MIWA, Kazumi NAKAMURA, Takeshi TSUNODA, Hidemasa KUWABARA, Yumiko FURUYA, Kunio DOBASHI, Masatomo MORI
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Keywords: trombiculid mites, genome, serotype
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2002 Volume 41 Issue 5 Pages 408-411

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Abstract

We treated a case of Tsutsugamushi disease diagnosed by polymerase chain reaction (PCR) using a scab specimen at the bite site of trombiculid mites. Otherwise the diagnosis could not be confirmed by serum antibody test nor the PCR test of blood. The genome of Rickettsia tsutsugamushi was detected and identified as the Kawasaki serotype strain. An attempt to extract the genome from the scab has not been reported, thus our data suggest that the scab is a useful specimen to confirm the diagnosis of Tsutsugamushi disease.
(Internal Medicine 41: 408-411, 2002)

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