The characteristics of non-metallic inclusions (such as number, size and volume fraction) in liquid steel samples taken during ladle treatment and casting of industrial heats of two low-alloyed Ca-treated steel grades were evaluated by using the Pulse Distribution Analysis with Optical Emission Spectroscopy (PDA/OES) method. These results were compared to data obtained by Scanning Electron Microscope observations of inclusions after electrolytic extraction from steel samples (the EE method). It was found that the PDA/OES method can be used for a relative estimation of the homogeneity of the distribution of non-metallic inclusions in steel samples. Bottom and middle parts of the steel samples showed more homogeneous results with respect to the characteristics of the investigated Al₂O₃, CaO–Al₂O₃ and CaO–Al₂O₃–CaS inclusions. The numbers of inclusions in the size ranges 2.0–5.7 µm and 1.4–5.7 µm in samples taken before and after a Ca addition, respectively, showed a relatively good agreement between both methods. Furthermore, the calculated volume fractions for inclusions in the size range 2–13 µm obtained by the PDA/OES method agreed satisfactorily well with those obtained from the EE method. Finally, the minimum sizes of inclusions in steel samples, which can reliably be detected by the PDA/OES method, were estimated for steels with different concentrations of Al in steel and Al₂O₃ in inclusions.