The aim of this study was to evaluate the influence of nanostructures on the surface of titanium, titanium nanosheets (TNS), on the initial attachment, proliferation, and gene expression of various functional factors by rat aortic endothelial cells (RAEC) involved in wound healing. We found that the initial attachment and proliferation of RAECs after 15, 30, 45, 60, and 120 min of culture were increased on titanium surfaces modified with TNS. We also found that the expression of intercellular adhesion molecule 1, von Willebrand, and thrombomodulin transcription factors were elevated in samples containing a TNS-modified disk in compared with unmodified titanium disks during the early stages of wound healing. Our results suggest that nanostructures on titanium surfaces stimulate RAECs to upregulate expression of angiogenic factors and adhesion molecule genes, which play an essential role in controlling inflammation and revascularization during wound healing after implantation.