Natural killer (NK) cells play a key role in inflammation and tumor regression through their ability to migrate into tissues. CXCL8 is one of the chemokines that promote leukocytes invasion and migration into tissues, while the exact molecular mechanisms are not clear at present. In this study, we showed that CXCL8 significantly enhanced CD16⁺CD56⁺ human peripheral NK cells degradation of type I collagen. MMP-1 associated with CXCL8-stimulated NK cell surface were co-localized with α2 integrin. The association of pro-MMP-1 with cell surface required a stimulation of CXCL8 in CD16⁺CD56⁺ human peripheral NK cells. Anti-MMP-1 antibody inhibited the degradation of type I collagen enhanced by CXCL8. Our data suggested that membrane associated MMP-1 plays a key role in the degradation of matrix proteins in CXCL8 activated CD16⁺CD56⁺ human peripheral NK cells. These results suggest that the selective regulations of production and/or localization of MMP-1 in NK cells may lead to effective strategies to control inflammation and tumor elimination.