Influence on the Long-term Differentiation Culture of ES-D3 Cells with String-like Collagen Scaffolds Derived from Tilapia Scales

Koichi IMAI; Tetsunari NISHIKAWA; Shosuke MORITA; Tomio ISEKI; Hiroaki YOSHIDA; Kazuhiro MATSUMOTO; Muneyasu SHIDA; Fumiya OGAWA; Kazuhiko SUESE

doi:10.11223/jarde.13.125

ORIGINAL ARTICLES

Influence on the Long-term Differentiation Culture of ES-D3 Cells with String-like Collagen Scaffolds Derived from Tilapia Scales

Koichi IMAI, Tetsunari NISHIKAWA, Shosuke MORITA, Tomio ISEKI, Hiroaki YOSHIDA, Kazuhiro MATSUMOTO, Muneyasu SHIDA, Fumiya OGAWA, Kazuhiko SUESE

Author information

Koichi IMAI
Department of Biomaterials,
Tetsunari NISHIKAWA
Department of Oral Pathology
Department of Innovation in Dental Education
Shosuke MORITA
First Department of Oral and Maxillofacial Surgery,
Tomio ISEKI
First Department of Oral and Maxillofacial Surgery,
Hiroaki YOSHIDA
First Department of Oral and Maxillofacial Surgery,
Kazuhiro MATSUMOTO
First Department of Oral and Maxillofacial Surgery,
Muneyasu SHIDA
Department of Endodontics,
Fumiya OGAWA
Department of Postgraduate Clinical Training,
Kazuhiko SUESE
Department of Esthetic Dentistry, Osaka Dental University, Osaka, Japan

Keywords: ES cells, marine collagen, porous collagen, caffold, Tilapia

JOURNAL FREE ACCESS

2015 Volume 13 Issue 3 Pages 125-130

DOI https://doi.org/10.11223/jarde.13.125

Details

Abstract

In the field of regenerative medicine, tissues and organs differentiated from iPS cells are implanted into the body. For this purpose, scaffolds using collagen derived from fish, evolutionarily distant from humans, are safer than those using mammalian (porcine) collagen. In the present study, the differentiation of mouse-derived ES cells was examined using string-like porous collagen derived from tilapia that inhabits tropical and subtropical regions. Cell differentiation into bone or cartilage requires 30-day culture. Whether or not the string-like collagen could withstand the long-term culture was examined, demonstrating that 86% of the collagen retained a gel form during the culture without any effect on ES cell differentiation.

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