Detection of Active Oxygen Generated from Ceramic Powders Having Antibacterial Activity

Jun Sawai; Emiko Kawada; Fumio Kanou; Hideo Igarashi; Atsushi Hashimoto; Takao Kokugan; Masaru Shimizu

doi:10.1252/jcej.29.627

Research Papers

Detection of Active Oxygen Generated from Ceramic Powders Having Antibacterial Activity

Jun Sawai, Emiko Kawada, Fumio Kanou, Hideo Igarashi, Atsushi Hashimoto, Takao Kokugan, Masaru Shimizu

Author information

Jun Sawai
Department of Chernical Engineering, Division of Applied Chemistry, Tokyo University of Agriculture & Technology
Emiko Kawada
Department of Chernical Engineering, Division of Applied Chemistry, Tokyo University of Agriculture & Technology
Fumio Kanou
Department of Chernical Engineering, Division of Applied Chemistry, Tokyo University of Agriculture & Technology
Hideo Igarashi
Department of Chernical Engineering, Division of Applied Chemistry, Tokyo University of Agriculture & Technology
Atsushi Hashimoto
Department of Chernical Engineering, Division of Applied Chemistry, Tokyo University of Agriculture & Technology
Takao Kokugan
Department of Chernical Engineering, Division of Applied Chemistry, Tokyo University of Agriculture & Technology
Masaru Shimizu
Department of Chernical Engineering, Division of Applied Chemistry, Tokyo University of Agriculture & Technology

Keywords: Biochemical Engineering, Ceramics, Antibacterial Activity, Active Oxygen, Chemiluminescene Analysis

JOURNAL FREE ACCESS

1996 Volume 29 Issue 4 Pages 627-633

DOI https://doi.org/10.1252/jcej.29.627

Details

Abstract

In order to elucidate the antibacterial mechanisms of magnesium oxide (MgO), calcium oxide (CaO) and zinc oxide (ZnO), the generation of active oxygen from these ceramic powder slurries was examined by oxygen electrode analysis and chemiluminescence analysis. Hydrogen peroxide (H₂O₂) generated from the ZnO powder slurry was detected using the oxygen electrode. Active oxygen from the MgO and CaO powder slurries was not detected by the oxygen electrode analysis. Chemiluminescence analysis could detect the generation of active oxygen from three kinds of powders. The luminescence response of the CaO powder slurry was markedly strong. The chemiluminescence responses of the CaO and MgO powder slurries are due to the superoxide anion (O₂^–). The order of the strength of luminescence response was CaO, MgO, and ZnO, which agreed with that of the antibacterial activity of these powders. These results suggested that active oxygen species generated from the ceramic powders were associated with their antibacterial activities.

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