Proteome analysis of silkworm 2. Hemolymph

Hideyuki Kajiwara; Atsue Imamaki; Masatoshi Nakamura; Kazuei Mita; Qingyu Xia; Masami Ishizaka

doi:10.2198/jelectroph.53.27

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Proteome analysis of silkworm 2. Hemolymph

Hideyuki Kajiwara, Atsue Imamaki, Masatoshi Nakamura, Kazuei Mita, Qingyu Xia, Masami Ishizaka

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Keywords: hemolymph, mass spectrometry, proteome, silkworm (Bombyx mori L.), two-dimensional polyacrylamide gel electrophoresis

JOURNAL FREE ACCESS

2009 Volume 53 Issue 2 Pages 27-31

DOI https://doi.org/10.2198/jelectroph.53.27

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Abstract

Proteomic analysis of silkworm hemolymph at the fifth-instar day-3 larva stage was performed by combination of two-dimensional polyacrylamide gel electrophoresis and mass spectrometry. Prominent spots on 2D-gel were analyzed after trypsinization and proteins were identified by Mascot and Sequest using the amino acid sequence data obtained from the genome sequence of silkworm. The gel images were tracked from the fourth-instar day-1 larva stage to the stage before adult moth for twenty-one days. The results of mass spectrometry analysis and changes in gel images are summarized in the silkworm proteome database (http://kaiko2ddb.dna.affrc.go.jp/cgi-bin/search_2DDB.cgi).

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