Isolation of lactic acid bacteria exhibiting high scavenging activity for environmental hydrogen peroxide from fermented foods and its two scavenging enzymes for hydrogen peroxide

Akio Watanabe; Chiaki Kaneko; Yasuhiro Hamada; Kouji Takeda; Shinya Kimata; Takashi Matsumoto; Akira Abe; Naoto Tanaka; Sanae Okada; Masataka Uchino; Junichi Satoh; Junichi Nakagawa; Youichi Niimura

doi:10.2323/jgam.62.75

Full Papers

Isolation of lactic acid bacteria exhibiting high scavenging activity for environmental hydrogen peroxide from fermented foods and its two scavenging enzymes for hydrogen peroxide

Akio Watanabe, Chiaki Kaneko, Yasuhiro Hamada, Kouji Takeda, Shinya Kimata, Takashi Matsumoto, Akira Abe, Naoto Tanaka, Sanae Okada, Masataka Uchino, Junichi Satoh, Junichi Nakagawa, Youichi Niimura

Author information

Akio Watanabe
Department of Bio-Science, Tokyo University of Agriculture
TAIYO Corporation, Laboratory
Chiaki Kaneko
Department of Bio-Science, Tokyo University of Agriculture
Yasuhiro Hamada
Department of Bio-Science, Tokyo University of Agriculture
Kouji Takeda
Department of Bio-Science, Tokyo University of Agriculture
Shinya Kimata
Department of Bio-Science, Tokyo University of Agriculture
Takashi Matsumoto
Nodai Genome Research Center, Tokyo University of Agriculture
Akira Abe
Department of Ophthalmology, Sapporo Medical University
Naoto Tanaka
Nodai Culture Collection Center, Tokyo University of Agriculture
Sanae Okada
Nodai Culture Collection Center, Tokyo University of Agriculture
Masataka Uchino
Department of Applied Biology and Chemistry, Tokyo University of Agriculture
Junichi Satoh
Department of Bio-Science, Tokyo University of Agriculture
Junichi Nakagawa
Department of Food Science and Technology, Tokyo University of Agriculture
Youichi Niimura
Department of Bio-Science, Tokyo University of Agriculture

Keywords: bacterial living cells, hydrogen peroxide, lactic acid bacteria, NADH peroxidase, scavenging of hydrogen peroxide

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Supplementary material

2016 Volume 62 Issue 2 Pages 75-82

DOI https://doi.org/10.2323/jgam.62.75

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Abstract

To obtain lactic acid bacteria that scavenge environmental hydrogen peroxide, we developed a specialized enrichment medium and successfully isolated Pediococcus pentosaceus Be1 strain from a fermented food. This strain showed vigorous environmental hydrogen peroxide scavenging activity over a wide range of hydrogen peroxide concentrations. High Mn-catalase and NADH peroxidase activities were found in the cell-free extract of the P. pentosaceus Be1 strain, and these two hydrogen peroxide scavenging enzymes were purified from the cell-free extract of the strain. Mn-catalase has been purified from several microorganisms by several researchers, and the NADH peroxidase was first purified from the original strain in this report. After cloning the genes of the Mn-catalase and the NADH peroxidase, the deduced amino acid sequences were compared with those of known related enzymes.

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