We reared three parasitoid species from field-collected or sentinel eggs of Glaucias subpunctatus (Walker), which is a serious pest of fruit trees in Japan. Morphological observation revealed that they were Trissolcus japonicus (Ashmead), T. plautiae (Watanabe) (Hymenoptera: Platygastridae) and Anastatus japonicus Ashmead (Hymenoptera: Eupelmidae). With regard to T. japonicus, the results of DNA analysis (a partial COI region of mtDNA) supported those of morphological identification. We did not analyze the DNA of T. plautiae and A. japonicus in this study due to a limited number of specimens. This is the first record of egg parasitoids that attack G. subpunctatus. The specimens examined in this study were kept in the collection of the Entomological Laboratory, Faculty of Agriculture, Kyushu University, Japan.