Little is known about the mechanism of tissue maintenance and regeneration for lingual epithelial cells. And specific stem cell markers of the lingual epithelium and the function have not much been reported so far. Multicolor lineage tracing method enables to distinguish the cell clusters derived from many different stem cells and clarifies the area derived from the long-term stem cells. Then in the area, we identify a single Bmi1 positive cell as specific lingual stem cells. By Bmi1-positive cells lineage tracing, for the first time we shows Bmi1-positive stem cells have maintained the lingual epithelium for long time and that can regenerate the injured lingual tissues.