Agroecosystem Development of Industrial Fermentation Waste —Characterization of Aroma-active Compounds from the Cultivation Medium of Lactobacillus brevis—

Toshirou Ono; Atsushi Usami; Satoshi Nakaya; Hideto Shinpuku; Yasunori Yonejima; Atsushi Ikeda; Mitsuo Miyazawa

doi:10.5650/jos.ess14257

General Subjects

Agroecosystem Development of Industrial Fermentation Waste —Characterization of Aroma-active Compounds from the Cultivation Medium of Lactobacillus brevis—

Toshirou Ono, Atsushi Usami, Satoshi Nakaya, Hideto Shinpuku, Yasunori Yonejima, Atsushi Ikeda, Mitsuo Miyazawa

Author information

Keywords: media after incubation (MAI), utilizing waste liquid, aroma extract dilution analysis (AEDA), Lactobacillus brevis

JOURNAL FREE ACCESS

2015 Volume 64 Issue 5 Pages 585-594

DOI https://doi.org/10.5650/jos.ess14257

Browse “Advance online publication” version

Details

Abstract

Volatile oils obtained from both the liquid medium after incubation (MAI) and liquid medium before incubation (MBI) during the cultivation process of Lactobacillus brevis were isolated by hydrodistillation (HD) and analyzed to determine the utility of the liquid waste. The composition of the volatile oils was analyzed by capillary gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). In total, 55 and 36 compounds were detected in the volatile oils from MAI (MAI oil) and MBI (MBI oil), respectively. The principle components of MAI oil were N-containing compounds, including 2,3-dimethylpyrazine (16, 37.1 %), methylpyrazine (4, 17.1 %). The important aroma-active compounds in the oils were detected by GC-Olfactometry (GC-O), and their intensity of aroma were measured by aroma extract dilution analysis (AEDA). Expressly, pyrazine compounds were determined as key aroma components; in particular, 2,5-dimethylpyrazine and 2,3-dimethylpyrazine were the most primary aroma-active compound in MAI oil. These results imply that the waste medium after incubation of L. brevis may be utilized as a source of volatile oils.

Corresponding author

Register with J-STAGE for free!