A huge deposit of buton natural asphalt in Buton island of South East Sulawesi, Indonesia, has not been fully utilized for asphalt concrete mixture. This is because the asphalt of buton granular asphalt is trapped in mineral, and currently the modifier to rejuvenate buton granular asphalt has not resulted in road pavement that can bear heavy daily traffic. This study aims to examine the performance of bio-flux oil as modifier of buton granular asphalt to determine its asphalt mixture requirement properties. This bio-flux oil consists of the oil derived from the seeds of the oil nut tree (Calophyllum inophyllum L.), crude pine resin from pine tree (Pinus merkusii), and other ingredients in certain composition.