Prolonged Esophagitis After Primary Dysfunction of the Pyloric Sphincter in the Rat and Therapeutic Potential of the Gastric Pentadecapeptide BPC 157

Ivan Dobric; Petar Drvis; Igor Petrovic; Drazen Shejbal; Luka Brcic; Alenka Boban Blagaic; Lovorka Batelja; Marko Sever; Neven Kokic; Ante Tonkic; Ivan Zoricic; Sandro Mise; Mario Staresinic; Bozo Radic; Ana Jakir; Jaksa Babel; Spomenko Ilic; Tihomir Vuksic; Ivan Jelic; Tomislav Anic; Sven Seiwerth; Predrag Sikiric

doi:10.1254/jphs.FP0061322

Full Papers

Prolonged Esophagitis After Primary Dysfunction of the Pyloric Sphincter in the Rat and Therapeutic Potential of the Gastric Pentadecapeptide BPC 157

Ivan Dobric, Petar Drvis, Igor Petrovic, Drazen Shejbal, Luka Brcic, Alenka Boban Blagaic, Lovorka Batelja, Marko Sever, Neven Kokic, Ante Tonkic, Ivan Zoricic, Sandro Mise, Mario Staresinic, Bozo Radic, Ana Jakir, Jaksa Babel, Spomenko Ilic, Tihomir Vuksic, Ivan Jelic, Tomislav Anic, Sven Seiwerth, Predrag Sikiric

Author information

Ivan Dobric
Department of Pharmacology, Medical School, University of Zagreb, Croatia
Petar Drvis
Department of Pharmacology, Medical School, University of Zagreb, Croatia
Igor Petrovic
Department of Pharmacology, Medical School, University of Zagreb, Croatia
Drazen Shejbal
Department of Pharmacology, Medical School, University of Zagreb, Croatia
Luka Brcic
Institute of Pathology, Medical School, University of Zagreb, Croatia
Alenka Boban Blagaic
Department of Pharmacology, Medical School, University of Zagreb, Croatia
Lovorka Batelja
Institute of Pathology, Medical School, University of Zagreb, Croatia
Marko Sever
Department of Pharmacology, Medical School, University of Zagreb, Croatia
Neven Kokic
Department of Pharmacology, Medical School, University of Zagreb, Croatia
Ante Tonkic
Department of Pharmacology, Medical School, University of Zagreb, Croatia
Ivan Zoricic
Department of Pharmacology, Medical School, University of Zagreb, Croatia
Sandro Mise
Department of Pharmacology, Medical School, University of Zagreb, Croatia
Mario Staresinic
Department of Pharmacology, Medical School, University of Zagreb, Croatia
Bozo Radic
Department of Pharmacology, Medical School, University of Zagreb, Croatia
Ana Jakir
Department of Pharmacology, Medical School, University of Zagreb, Croatia
Jaksa Babel
Department of Pharmacology, Medical School, University of Zagreb, Croatia
Spomenko Ilic
Department of Pharmacology, Medical School, University of Zagreb, Croatia
Tihomir Vuksic
Department of Pharmacology, Medical School, University of Zagreb, Croatia
Ivan Jelic
Department of Pharmacology, Medical School, University of Zagreb, Croatia
Tomislav Anic
Department of Pharmacology, Medical School, University of Zagreb, Croatia
Sven Seiwerth
Institute of Pathology, Medical School, University of Zagreb, Croatia
Predrag Sikiric
Department of Pharmacology, Medical School, University of Zagreb, Croatia

Keywords: stable gastric pentadecapeptide BPC 157, ranitidine, pyloric sphincter dysfunction, sphincter pressure, rat esophagitis

JOURNAL FREE ACCESS

2007 Volume 104 Issue 1 Pages 7-18

DOI https://doi.org/10.1254/jphs.FP0061322

View "Advance Publication" version (April 24, 2007).

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Abstract

Seven or fourteen days or twelve months after suturing one tube into the pyloric sphincter (removed by peristalsis by the seventh day), rats exhibit prolonged esophagitis with a constantly lowered pressure not only in the pyloric, but also in the lower esophageal sphincter and a failure of both sphincters. Throughout the esophagitis experiment, gastric pentadecapeptide BPC 157 (PL 14736) is given intraperitoneally once a day (10 μg/kg, 10 ng/kg, last application 24 h before assessment), or continuously in drinking water at 0.16 μg/ml, 0.16 ng/ml (12 ml/rat per day), or directly into the stomach 5 min before pressure assessment (a water manometer connected to the drainage port of a Foley catheter implanted into the stomach either through an esophageal or duodenal incision). This treatment alleviates i) the esophagitis (macroscopically and microscopically, at either region or interval), ii) the pressure in the pyloric sphincter, and iii) the pressure in the lower esophageal sphincter (cmH₂O). In the normal rats it increases lower esophageal sphincter pressure, but decreases the pyloric sphincter pressure. Ranitidine, given using the same protocol (50 mg/kg, intraperitoneally, once daily; 0.83 mg/ml in drinking water; 50 mg/kg directly into the stomach) does not have an effect in either rats with esophagitis or in normal rats.

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