Short Communications
Protein O-Glycosylation Induces Collagen Expression and Contributes to Diabetic Cardiomyopathy in Rat Cardiac Fibroblasts
2009 Volume 111 Issue 4 Pages 446-450
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2009 Volume 111 Issue 4 Pages 446-450
Diabetic cardiomyopathy may be accompanied by myocardial fibrosis. We have previously reported that cardiac fibrosis and protein O-glycosylation are elevated in diabetes. In this study, we examined if the hexosamine biosynthesis pathway (HBP) was involved with collagen expression in rat cardiac fibroblasts (RCFs). Long-term glucose load significantly increased type III collagen expression in RCFs, but did not affect the protein O-glycosylation. In addition, glucosamine treatment not only induced expressions of collagen types I and III, but also increased the O-glycosylated protein. These results suggest that O-glycosylation of protein induced by HBP activation modifies collagen expression and contributes to diabetic cardiomyopathy.
