The Cannabinoid 1–Receptor Silent Antagonist O-2050 Attenuates Preference for High-Fat Diet and Activated Astrocytes in Mice

Sei Higuchi; Keiichi Irie; Shohei Mishima; Maiko Araki; Makiko Ohji; Atsunori Shirakawa; Yoshiharu Akitake; Kiyoshi Matsuyama; Kenji Mishima; Kenichi Mishima; Katsunori Iwasaki; Michihiro Fujiwara

doi:10.1254/jphs.09326SC

Short Communications

The Cannabinoid 1–Receptor Silent Antagonist O-2050 Attenuates Preference for High-Fat Diet and Activated Astrocytes in Mice

Sei Higuchi, Keiichi Irie, Shohei Mishima, Maiko Araki, Makiko Ohji, Atsunori Shirakawa, Yoshiharu Akitake, Kiyoshi Matsuyama, Kenji Mishima, Kenichi Mishima, Katsunori Iwasaki, Michihiro Fujiwara

Author information

Sei Higuchi
Department of Neuropharmacology, Faculty of Pharmaceutical Sciences, Fukuoka University, Japan
Keiichi Irie
Department of Neuropharmacology, Faculty of Pharmaceutical Sciences, Fukuoka University, Japan
Advanced Materials Institute, Fukuoka University, Japan
Shohei Mishima
Department of Neuropharmacology, Faculty of Pharmaceutical Sciences, Fukuoka University, Japan
Maiko Araki
Department of Neuropharmacology, Faculty of Pharmaceutical Sciences, Fukuoka University, Japan
Makiko Ohji
Department of Neuropharmacology, Faculty of Pharmaceutical Sciences, Fukuoka University, Japan
Atsunori Shirakawa
Department of Neuropharmacology, Faculty of Pharmaceutical Sciences, Fukuoka University, Japan
Yoshiharu Akitake
Department of Neuropharmacology, Faculty of Pharmaceutical Sciences, Fukuoka University, Japan
Advanced Materials Institute, Fukuoka University, Japan
Kiyoshi Matsuyama
Department of Chemical Engineering, Faculty of Engineering, Fukuoka University, Japan
Kenji Mishima
Department of Chemical Engineering, Faculty of Engineering, Fukuoka University, Japan
Kenichi Mishima
Department of Neuropharmacology, Faculty of Pharmaceutical Sciences, Fukuoka University, Japan
Advanced Materials Institute, Fukuoka University, Japan
Katsunori Iwasaki
Department of Neuropharmacology, Faculty of Pharmaceutical Sciences, Fukuoka University, Japan
Advanced Materials Institute, Fukuoka University, Japan
Michihiro Fujiwara
Department of Neuropharmacology, Faculty of Pharmaceutical Sciences, Fukuoka University, Japan

Keywords: preference for high-fat diet, endocannabinoid, astrocyte

JOURNAL FREE ACCESS

2010 Volume 112 Issue 3 Pages 369-372

DOI https://doi.org/10.1254/jphs.09326SC

View "Advance Publication" version (February 18, 2010).

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Abstract

Endocannabinoids have been shown to activate reward-related feeding and to promote astrocytic differentiation. We investigated whether high-fat diet (HFD) intake produced a preference for HFD via an endocannabinoid-dependent mechanism. In the conditioned place preference test, the 2-week HFD–intake group showed preference for HFD and had increased expression of a marker for reactive astrocytes, glial fibrillary acid protein (GFAP), in the hypothalamus. The cannabinoid CB₁–receptor antagonist O-2050 reduced the preference for HFD and expression of GFAP in the hypothalamus. These results suggested that HFD intake led to the development of a preference for HFD via astrocytic CB₁ receptors in the hypothalamus.

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