Harpagophytum procumbens Suppresses Lipopolysaccharide-Stimulated Expressions of Cyclooxygenase-2 and Inducible Nitric Oxide Synthase in Fibroblast Cell Line L929

Mi-Hyeon Jang; Sabina Lim; Seung-Moo Han; Hi-Joon Park; Insop Shin; Jin-Woo Kim; Nam-Jae Kim; Ji-Suk Lee; Kyung-Ah Kim; Chang-Ju Kim

doi:10.1254/jphs.93.367

Short Communications

Harpagophytum procumbens Suppresses Lipopolysaccharide-Stimulated Expressions of Cyclooxygenase-2 and Inducible Nitric Oxide Synthase in Fibroblast Cell Line L929

Mi-Hyeon Jang, Sabina Lim, Seung-Moo Han, Hi-Joon Park, Insop Shin, Jin-Woo Kim, Nam-Jae Kim, Ji-Suk Lee, Kyung-Ah Kim, Chang-Ju Kim

Author information

Mi-Hyeon Jang
Department of Physiology, College of Medicine, Kyung Hee University
Sabina Lim
Research Group of Pain and Neuroscience in Vision 2000 Project, East-west Medical Research Institute, Kyung Hee University
Seung-Moo Han
Research Group of Pain and Neuroscience in Vision 2000 Project, East-west Medical Research Institute, Kyung Hee University
Hi-Joon Park
Research Group of Pain and Neuroscience in Vision 2000 Project, East-west Medical Research Institute, Kyung Hee University
Insop Shin
Research Group of Pain and Neuroscience in Vision 2000 Project, East-west Medical Research Institute, Kyung Hee University
Jin-Woo Kim
Research Group of Pain and Neuroscience in Vision 2000 Project, East-west Medical Research Institute, Kyung Hee University
Nam-Jae Kim
Research Group of Pain and Neuroscience in Vision 2000 Project, East-west Medical Research Institute, Kyung Hee University
Ji-Suk Lee
Research Group of Pain and Neuroscience in Vision 2000 Project, East-west Medical Research Institute, Kyung Hee University
Kyung-Ah Kim
Research Group of Pain and Neuroscience in Vision 2000 Project, East-west Medical Research Institute, Kyung Hee University
Chang-Ju Kim
Department of Physiology, College of Medicine, Kyung Hee University
Research Group of Pain and Neuroscience in Vision 2000 Project, East-west Medical Research Institute, Kyung Hee University

Keywords: Harpagophytum procumbens, lipopolysaccharide, cyclooxygenase-2

JOURNAL FREE ACCESS

2003 Volume 93 Issue 3 Pages 367-371

DOI https://doi.org/10.1254/jphs.93.367

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Abstract

Harpagophytum procumbens (Pedaliaceae) has been used for the treatment of pain and arthritis. The effect of Harpagophytum procumbens against lipopolysaccharide-induced inflammation was investigated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, reverse transcription-polymerase chain reaction, prostaglandin E₂ (PGE₂) immunoassay, and nitric oxide detection on mouse fibroblast cell line L929. The aqueous extract of Harpagophytum procumbens was shown to suppress PGE₂ synthesis and nitric oxide production by inhibiting lipopolysaccharide-stimulated enhancement of the cyclooxygenase-2 and inducible nitric oxide synthase (iNOS) mRNAs expressions in L929 cells. These results suggest that Harpagophytum procumbens exerts anti-inflammatory and analgesic effects probably by suppressing cyclooxygenase-2 and iNOS expressions.

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