[Purpose] This study was conducted to examine physical status and life satisfaction of workers in Korea. [Subjects] In total, 25,455 workers from eight geographical areas were evaluated from 2007 to 2008. [Methods] Physical status of the subjects was measured based on cardiopulmonary endurance, muscular strength, muscular endurance, flexibility, and explosive muscular strength, and a life satisfaction index was used to measure life satisfaction. [Results] Higher levels of cardiopulmonary endurance, muscular strength, muscular endurance, flexibility, and explosive muscular strength of workers were correlated with higher life satisfaction. [Conclusion] The result suggests it is necessary to improve life satisfaction through continuous health management of workers.