Both PPARγ and C/EBPα are Sufficient to Induce Transdifferentiation of Goat Fetal Myoblasts into Adipocytes

Keitaro YAMANOUCHI; Ayako BAN; Shingo SHIBATA; Tohru HOSOYAMA; Yousuke MURAKAMI; Masugi NISHIHARA

doi:10.1262/jrd.18169

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Both PPARγ and C/EBPα are Sufficient to Induce Transdifferentiation of Goat Fetal Myoblasts into Adipocytes

Keitaro YAMANOUCHI, Ayako BAN, Shingo SHIBATA, Tohru HOSOYAMA, Yousuke MURAKAMI, Masugi NISHIHARA

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Keywords: Adipogenesis, CCAAT/enhancer binding protein-α (C/EBPα), Goat, Myoblast, Peroxisome proliferator activating receptor-γ (PPARγ), Transdifferentiation

JOURNAL FREE ACCESS

2007 Volume 53 Issue 3 Pages 563-572

DOI https://doi.org/10.1262/jrd.18169

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Abstract

The present study was conducted to investigate whether goat fetal myoblasts with no inherent adipogenic potential can be induced to transdifferentiate into adipocytes. Goat fetal myoblasts were transiently transfected by the adipogenic transcription factors, peroxisome proliferator-activated receptor-γ (PPARγ) and CCAAT/enhancer-binding protein-α (C/EBPα). Both PPARγ and C/EBPα were capable of inducing adipogenic transdifferentiation as indicated by the appearance of mature adipocytes when the transfected cells were cultured in adipogenic differentiation medium (ADM). Ectopic expression of PPARγ induced endogenous C/EBPα expression and vice versa only when the cells were cultured in ADM. Removal of troglitazone, a PPARγ agonist, from the ADM resulted in a dramatic decline in the number of adipocytes, indicating that PPARγ stimulation is necessary to induce adipogenic transdifferentiation of goat fetal myoblasts. These results demonstrate for the first time that primary cultured myoblasts can be transdifferentiated into adipocytes.

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