Protective Effect of Prostaglandin E1 on Radiation-Induced Proliferative Inhibition and Apoptosis in Keratinocytes and Healing of Radiation-Induced Skin Injury in Rats

Megumi TAKIKAWA; Yuki SUMI; Yoshihiro TANAKA; Masaki NAMBU; Takashi DOUMOTO; Satoshi YANAGIBAYASHI; Ryuichi AZUMA; Naoto YAMAMOTO; Satoko KISHIMOTO; Masayuki ISHIHARA; Tomoharu KIYOSAWA

doi:10.1269/jrr.11193

Biology

Protective Effect of Prostaglandin E₁ on Radiation-Induced Proliferative Inhibition and Apoptosis in Keratinocytes and Healing of Radiation-Induced Skin Injury in Rats

Megumi TAKIKAWA, Yuki SUMI, Yoshihiro TANAKA, Masaki NAMBU, Takashi DOUMOTO, Satoshi YANAGIBAYASHI, Ryuichi AZUMA, Naoto YAMAMOTO, Satoko KISHIMOTO, Masayuki ISHIHARA, Tomoharu KIYOSAWA

Author information

Megumi TAKIKAWA
Department of Plastic Surgery, National Defense Medical College
Yuki SUMI
Department of Plastic Surgery, National Defense Medical College
Yoshihiro TANAKA
Division of Biomedical Engineering, Research Institute, National Defense Medical College
Masaki NAMBU
Department of Plastic Surgery, National Defense Medical College
Takashi DOUMOTO
Department of Plastic Surgery, National Defense Medical College
Satoshi YANAGIBAYASHI
Department of Plastic Surgery, National Defense Medical College
Ryuichi AZUMA
Department of Plastic Surgery, National Defense Medical College
Naoto YAMAMOTO
Department of Plastic Surgery, National Defense Medical College
Satoko KISHIMOTO
Division of Biomedical Engineering, Research Institute, National Defense Medical College
Reseach Fellow of the Japan Society for Promotion of Science
Masayuki ISHIHARA
Division of Biomedical Engineering, Research Institute, National Defense Medical College
Tomoharu KIYOSAWA
Department of Plastic Surgery, National Defense Medical College

Keywords: Radioprotection, Prostaglandin E₁, Apoptosis, Keratinocytes, Healing-impaired wound

JOURNAL OPEN ACCESS

2012 Volume 53 Issue 3 Pages 385-394

DOI https://doi.org/10.1269/jrr.11193

Details

Abstract

We examined the effects of prostaglandin E₁ (PGE₁) on radiation-induced proliferation inhibition and apoptosis in keratinocytes and healing of radiation-induced skin injury in a rat model. PGE₁ had a protective effect on radiation-induced growth inhibition in keratinocytes in vitro, but not in fibroblasts. Varying concentrations of PGE₁ were subcutaneously administered into the posterior neck region. X-irradiation at a dose of 20 Gy was administrated to the lower part of the back using a lead sheet with two holes 30 min to 1 h before or after the administration of PGE₁. Although X-irradiation induced epilation, minor erosions, or skin ulcers in almost all rats, PGE₁ administration prior to irradiation reduced these irradiation injuries. Staining with terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling showed that proportions of apoptotic keratinocytes in the X-irradiated skin of PGE₁-administered rats were significantly lower than for those in the skin of rats which did not receive PGE₁. Cutaneous full-thickness defective wounds were then formed in X-irradiated areas to examine the time course of wound healing. Wound healing was significantly delayed because of X-irradiation, but PGE₁ administration prior to irradiation led to a significantly shorter delay in wound healing compared with controls. Decreasing delay in wound healing was correlated with concentration of PGE₁ administrated. Thus, PGE₁-administration may potentially alleviate the radiation-induced skin injury.

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