Effects of Hemagglutination Activity in the Serum of a Deep-Sea Vent Endemic Crab, Shinkaia Crosnieri, on Non-Symbiotic and Symbiotic Bacteria

So Fujiyoshi; Hiroaki Tateno; Tomoo Watsuji; Hideyuki Yamaguchi; Daisuke Fukushima; Sayaka Mino; Makoto Sugimura; Tomoo Sawabe; Ken Takai; Shigeki Sawayama; Satoshi Nakagawa

doi:10.1264/jsme2.ME15066

Regular Papers

Effects of Hemagglutination Activity in the Serum of a Deep-Sea Vent Endemic Crab, Shinkaia Crosnieri, on Non-Symbiotic and Symbiotic Bacteria

So Fujiyoshi, Hiroaki Tateno, Tomoo Watsuji, Hideyuki Yamaguchi, Daisuke Fukushima, Sayaka Mino, Makoto Sugimura, Tomoo Sawabe, Ken Takai, Shigeki Sawayama, Satoshi Nakagawa

Author information

So Fujiyoshi
Laboratory of Marine Environmental Microbiology, Graduate School of Agriculture, Kyoto University
Hiroaki Tateno
Research Center for Stem Cell Engineering, National Institute of Advanced Industrial Science and Technology (AIST)
Tomoo Watsuji
Subsurface Geobiology and Advanced Research project (SUGAR), Institute of Biogeosciences, Japan Agency for Marine-Earth Science and Technology (JAMSTEC)
Hideyuki Yamaguchi
Leica Microsystems K.K. Osaka Sales Office Shogyo
Daisuke Fukushima
Leica Microsystems K.K. Osaka Sales Office Shogyo
Sayaka Mino
Laboratory of Microbiology, Faculty of Fisheries Sciences, Hokkaido University
Makoto Sugimura
Enoshima Aquarium
Tomoo Sawabe
Laboratory of Microbiology, Faculty of Fisheries Sciences, Hokkaido University
Ken Takai
Subsurface Geobiology and Advanced Research project (SUGAR), Institute of Biogeosciences, Japan Agency for Marine-Earth Science and Technology (JAMSTEC)
Shigeki Sawayama
Laboratory of Marine Environmental Microbiology, Graduate School of Agriculture, Kyoto University
Satoshi Nakagawa
Laboratory of Marine Environmental Microbiology, Graduate School of Agriculture, Kyoto University
Subsurface Geobiology and Advanced Research project (SUGAR), Institute of Biogeosciences, Japan Agency for Marine-Earth Science and Technology (JAMSTEC)

Keywords: Hemagglutinin/lectin, Deep-sea hydrothermal field, Self/non-self recognition, Host-microbe interaction, Symbiosis

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Supplementary material

2015 Volume 30 Issue 3 Pages 228-234

DOI https://doi.org/10.1264/jsme2.ME15066

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Abstract

In deep-sea hydrothermal environments, most invertebrates associate with dense populations of symbiotic microorganisms in order to obtain nutrition. The molecular interactions between deep-sea animals and environmental microbes, including their symbionts, have not yet been elucidated in detail. Hemagglutinins/lectins, which are carbohydrate-binding proteins, have recently been reported to play important roles in a wide array of biological processes, including the recognition and control of non-self materials. We herein assessed hemagglutination activity in the serum of a deep-sea vent endemic crab, Shinkaia crosnieri, which harbors chemosynthetic epibionts on its plumose setae. Horse and rabbit erythrocytes were agglutinated using this serum (opt. pH 7.5 and opt. temperature 15°C). Agglutinating activity was inhibited by eight kinds of sugars and several divalent cations, did not require any divalent metal ions, and remained detectable even after heating the serum at 100°C for 30 min. By using fluorescently labeled serum, we demonstrated that deep-sea crab serum components bound to the epibionts even in the presence of sugars. This study represents the first immunological assessment of a deep-sea vent endemic crab and demonstrated the possibility of a non-lectin-mediated symbiont-host interaction.

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