Characterization and tissue distribution of conjugated metabolites of pyrene in the rat

Aksorn SAENGTIENCHAI; Yoshinori IKENAKA; Wageh Sobhy DARWISH; Shouta M.M. NAKAYAMA; Hazuki MIZUKAWA; Mayumi ISHIZUKA

doi:10.1292/jvms.14-0632

Toxicology

Characterization and tissue distribution of conjugated metabolites of pyrene in the rat

Aksorn SAENGTIENCHAI, Yoshinori IKENAKA, Wageh Sobhy DARWISH, Shouta M.M. NAKAYAMA, Hazuki MIZUKAWA, Mayumi ISHIZUKA

Author information

Aksorn SAENGTIENCHAI
Laboratory of Toxicology, Department of Environmental Veterinary Sciences, Graduate School of Veterinary Medicine, Hokkaido University, N18 W9, Kita-ku, Sapporo 060–0818, Japan
Department of Pharmacology, Faculty of Veterinary Medicine, Kasetsart University, 50 Ngam Wong Wan Rd, Lat Yao Chatuchak, Bangkok 10900, Thailand
Yoshinori IKENAKA
Laboratory of Toxicology, Department of Environmental Veterinary Sciences, Graduate School of Veterinary Medicine, Hokkaido University, N18 W9, Kita-ku, Sapporo 060–0818, Japan
Water Research Group, Unit for Environmental Sciences and Management, North-West University, Potchefstroom, South Africa
Wageh Sobhy DARWISH
Laboratory of Toxicology, Department of Environmental Veterinary Sciences, Graduate School of Veterinary Medicine, Hokkaido University, N18 W9, Kita-ku, Sapporo 060–0818, Japan
Food Control Department, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44519, Egypt
Shouta M.M. NAKAYAMA
Laboratory of Toxicology, Department of Environmental Veterinary Sciences, Graduate School of Veterinary Medicine, Hokkaido University, N18 W9, Kita-ku, Sapporo 060–0818, Japan
Hazuki MIZUKAWA
Department of Environmental Veterinary Sciences, Graduate School of Veterinary Medicine, Hokkaido University, N18 W9, Kita-ku, Sapporo 060–0818, Japan
Mayumi ISHIZUKA
Laboratory of Toxicology, Department of Environmental Veterinary Sciences, Graduate School of Veterinary Medicine, Hokkaido University, N18 W9, Kita-ku, Sapporo 060–0818, Japan

Keywords: conjugated metabolites, glucuronidation, rat, sulfation, tissue distribution

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Supplementary material

2015 Volume 77 Issue 10 Pages 1261-1267

DOI https://doi.org/10.1292/jvms.14-0632

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Abstract

Pyrene (PY) is a polycyclic aromatic hydrocarbon (PAH) that is often used as a biomarker for human and wildlife exposure to PAHs. As the metabolites of PAHs, similar to their parent compounds, pose public health risks, it is necessary to study their characteristics and tissue-specific distribution. The present study was performed to experimentally characterize PY metabolites and analyze the tissue-specific distribution of the conjugated metabolites after oral administration of PY to rats. PY metabolites, such as pyrenediol-disulfate (PYdiol-diS), pyrenediol-sulfate (PYdiol-S), pyrene-1-sufate (PYOS), pyrene-1-glucuronide (PYOG) and 1-hydroxypyrene (PYOH), were detected in rat urine. Although glucuronide conjugate was the predominant metabolite, the metabolite composition varied among tissues. Interestingly, the proportion of PYOH was high in the large intestine. Furthermore, PYOH was the only PY metabolite detected in feces.

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