Detection of the staphylococcal enterotoxin D-like gene from staphylococcal food poisoning isolates over the last two decades in Tokyo

Yasunori SUZUKI; Makiko KOBAYASHI; Shigeru MATSUSHITA; Satomi UEHARA; Rei KATO; Yusuke SATO’O; Hisaya K. ONO; Kenji SADAMASU; Akemi KAI; Yoichi KAMATA

doi:10.1292/jvms.15-0028

Public Health

Detection of the staphylococcal enterotoxin D-like gene from staphylococcal food poisoning isolates over the last two decades in Tokyo

Yasunori SUZUKI, Makiko KOBAYASHI, Shigeru MATSUSHITA, Satomi UEHARA, Rei KATO, Yusuke SATO’O, Hisaya K. ONO, Kenji SADAMASU, Akemi KAI, Yoichi KAMATA

Author information

Yasunori SUZUKI
Department of Veterinary Medicine, Faculty of Agriculture, Iwate University, 3–18–8 Ueda, Morioka-shi, Iwate 020–8550, Japan
The United Graduate School of Veterinary Sciences, Gifu University, 1–1 Yanagido, Gifu-shi, Gifu 501–1193, Japan
Department of Microbiology, Tokyo Metropolitan Institute of Public Health, 3–24–1 Hyakunin-cho, Shinjuku-ku, Tokyo 169–0073, Japan
Makiko KOBAYASHI
Department of Microbiology, Tokyo Metropolitan Institute of Public Health, 3–24–1 Hyakunin-cho, Shinjuku-ku, Tokyo 169–0073, Japan
Shigeru MATSUSHITA
Department of Microbiology, Tokyo Metropolitan Institute of Public Health, 3–24–1 Hyakunin-cho, Shinjuku-ku, Tokyo 169–0073, Japan
Satomi UEHARA
Department of Microbiology, Tokyo Metropolitan Institute of Public Health, 3–24–1 Hyakunin-cho, Shinjuku-ku, Tokyo 169–0073, Japan
Rei KATO
Department of Microbiology, Tokyo Metropolitan Institute of Public Health, 3–24–1 Hyakunin-cho, Shinjuku-ku, Tokyo 169–0073, Japan
Yusuke SATO’O
Department of Bacteriology, Hiroshima University Graduate School of Biomedical & Health Sciences, 1–2–3 Kasumi, Minami-ku, Hiroshima-shi, Hiroshima 734–8551, Japan
Hisaya K. ONO
Department of Microbiology and Immunology, Hirosaki University Graduate School of Medicine, 5 Zaifu-cho, Hirosaki-shi, Aomori 036–8562, Japan
Kenji SADAMASU
Department of Microbiology, Tokyo Metropolitan Institute of Public Health, 3–24–1 Hyakunin-cho, Shinjuku-ku, Tokyo 169–0073, Japan
Akemi KAI
Department of Microbiology, Tokyo Metropolitan Institute of Public Health, 3–24–1 Hyakunin-cho, Shinjuku-ku, Tokyo 169–0073, Japan
Yoichi KAMATA
Department of Veterinary Medicine, Faculty of Agriculture, Iwate University, 3–18–8 Ueda, Morioka-shi, Iwate 020–8550, Japan
The United Graduate School of Veterinary Sciences, Gifu University, 1–1 Yanagido, Gifu-shi, Gifu 501–1193, Japan

Keywords: mutant SED, plasmid, staphylococcal enterotoxin, staphylococcal food poisoning, Staphylococcus aureus

JOURNAL FREE ACCESS

2015 Volume 77 Issue 8 Pages 905-911

DOI https://doi.org/10.1292/jvms.15-0028

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Abstract

The plasmid is a very well-known mobile genetic element that participates in the acquisition of virulence genes, such as staphylococcal enterotoxins (SEs), via horizontal transfer. SEs are emetic toxins and causative agents in staphylococcal food poisoning (SFP). We herein identified the types of plasmids harbored by seven SFP isolates and examined their production of plasmid-related SE/SEl to determine whether the new types of plasmid-related SE or SE-like (SEl) toxins (i.e. SElJ and SER) were involved in SFP. These isolates harbored pIB485-like plasmids, and all, except for one isolate, produced SElJ and SER. The amount of SER produced by each isolate accounted for the highest or second highest percentage of the total amount of SE/SEl produced. These new types of plasmid-related SE/SEls as well as classical SE may play a role in SFP. The seven isolates were classified into two SED-production types; a high SED-production type (>500 ng/ml) and no SED-production type. A nucleotide sequencing analysis revealed that three plasmids harbored by the SED-non-producing isolates had a single-base deletion in the sed gene with a resulting stop codon (from 233 amino acids of the intact SED to 154 amino acids of the mutant SED (mSED)). A real-time reverse transcription-PCR analysis showed that the mRNA of the msed gene was transcribed in the isolates. If the msed gene was translated as a protein, mSED may act as an emetic toxin instead of intact SED.

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