Phylogenetic analysis of Newcastle disease viruses isolated from wild birds          in the Poyang Lake region of China

Shengtao FAN; Tiecheng WANG; Xiaolong GAO; Ying YING; Xue LI; Yongcheng LI; Yuanguo LI; Jinzhu MA; Heting SUN; Dong CHU; Yu XU; Songtao YANG; Qihan LI; Yuwei GAO; Xianzhu XIA

doi:10.1292/jvms.14-0080

Virology

Phylogenetic analysis of Newcastle disease viruses isolated from wild birds in the Poyang Lake region of China

Shengtao FAN, Tiecheng WANG, Xiaolong GAO, Ying YING, Xue LI, Yongcheng LI, Yuanguo LI, Jinzhu MA, Heting SUN, Dong CHU, Yu XU, Songtao YANG, Qihan LI, Yuwei GAO, Xianzhu XIA

Author information

Shengtao FAN
Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming 650118, PR China
Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Sciences, No. 666, Liuying Xilu, Jingyue Economic Development Zone, Changchun 130122, PR China
Tiecheng WANG
Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Sciences, No. 666, Liuying Xilu, Jingyue Economic Development Zone, Changchun 130122, PR China
Xiaolong GAO
State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, PR China
Ying YING
College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, PR China
Xue LI
Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Sciences, No. 666, Liuying Xilu, Jingyue Economic Development Zone, Changchun 130122, PR China
Yongcheng LI
General Station for surveillance of wildlife Diseases & Wildlife Borne Disease, State Forestry Administration, Shenyang 110086, PR China
Yuanguo LI
Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Sciences, No. 666, Liuying Xilu, Jingyue Economic Development Zone, Changchun 130122, PR China
Jinzhu MA
Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Sciences, No. 666, Liuying Xilu, Jingyue Economic Development Zone, Changchun 130122, PR China
Heting SUN
General Station for surveillance of wildlife Diseases & Wildlife Borne Disease, State Forestry Administration, Shenyang 110086, PR China
Dong CHU
General Station for surveillance of wildlife Diseases & Wildlife Borne Disease, State Forestry Administration, Shenyang 110086, PR China
Yu XU
General Station for surveillance of wildlife Diseases & Wildlife Borne Disease, State Forestry Administration, Shenyang 110086, PR China
Songtao YANG
Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Sciences, No. 666, Liuying Xilu, Jingyue Economic Development Zone, Changchun 130122, PR China
Qihan LI
Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming 650118, PR China
Yuwei GAO
Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Sciences, No. 666, Liuying Xilu, Jingyue Economic Development Zone, Changchun 130122, PR China
Xianzhu XIA
Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Sciences, No. 666, Liuying Xilu, Jingyue Economic Development Zone, Changchun 130122, PR China

Keywords: genotype, Newcastle disease virus, phylogenetic analysis, Poyang Lake

JOURNAL FREE ACCESS
Supplementary material

2015 Volume 77 Issue 9 Pages 1143-1149

DOI https://doi.org/10.1292/jvms.14-0080

Browse “Advance online publication” version

Details

Abstract

Newcastle disease virus (NDV) causes a highly contagious viral disease in poultry and wild birds, and it can cause significant economic loss worldwide. Eight viral strains were isolated by inoculating embryonated chicken eggs from the Poyang Lake region of China with swab samples. All eight of the NDV isolates were identified as class I genotype 3 strains, but they diverged notablely from class II viruses. Further analysis revealed that all eight NDV isolates were lentogenic strains containing the ¹¹²ERQER↓L¹¹⁷ motif at the F protein cleavage site. The strains were highly identical and were more species specific (chicken and waterfowl) than site specific (Nanchang and Duchang regions). The close phylogenetic proximity of these isolates indicates that viral transmission may happen between poultry and wild birds. Our study demonstrates that lentogenic class I NDVs exist in clinically healthy wild waterfowl and poultry within the Poyang Lake region. Active surveillance of these viruses to determine their evolution and origin is one of the most realistic strategies for preventing and controlling NDV outbreaks.

Corresponding author

Register with J-STAGE for free!