Production of Term Offspring by In Vitro Fertilization Using Old Mouse Spermatozoa

Teruhiko WAKAYAMA; Kentaro TANEMURA; Junichi SUTO; Kenkichi IMAMURA; Katsuhiro FUKUTA; Hidenori MORI; Kazunao KURAMOTO; Masamichi KUROHMARU; Yoshihiro HAYASHI

doi:10.1292/jvms.57.545

Teruhiko WAKAYAMA, Kentaro TANEMURA, Junichi SUTO, Kenkichi IMAMURA, Katsuhiro FUKUTA, Hidenori MORI, Kazunao KURAMOTO, Masamichi KUROHMARU, Yoshihiro HAYASHI

Author information

Teruhiko WAKAYAMA
Kentaro TANEMURA
Junichi SUTO
Kenkichi IMAMURA
Katsuhiro FUKUTA
Hidenori MORI
Kazunao KURAMOTO
Masamichi KUROHMARU
Yoshihiro HAYASHI
Author's Organization：
Department of Veterinary Anatomy, Faculty of Agriculture, The University of Tokyo
Department of Veterinary Anatomy, Faculty of Agriculture, The University of Tokyo
National Institute of Animal Health
National Institute of Animal Health
National Institute of Animal Health
Faculty of Agriculture, Ibaraki University
Tokyo Metropolitan Institute of Gerontology
Department of Veterinary Anatomy, Faculty of Agriculture, The University of Tokyo
Department of Veterinary Anatomy, Faculty of Agriculture, The University of Tokyo

Keywords: aging, fertilization, spermatozoa

JOURNAL FREE ACCESS

1995 Volume 57 Issue 3 Pages 545-547

DOI https://doi.org/10.1292/jvms.57.545

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Abstract

We successfully produced offspring of old male BDF₁ mice using in vitro fertilization. Although 7 old male mice (33 months of age) were infertile and revealed frequent degeneration in the seminiferous epithelia, 4 of them had spermatozoa in the caudae epididymides. The IVF rate of their sperm with eggs from young ICR mice was very high (82.0%). The production rate of offspring was also satisfactory (61.6%). The present study indicates that the IVF method is useful for producing offspring of aged male mice.

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