2014 Volume 56 Pages 43-47
To isolate Pseudomonas syringae pv. japonica (Psj) from wheat seeds, we surface-sterilized seeds in 1.5% sodium hypochlorite for 3 min and inserted them in selective medium to be cultured for 7 days at 25°C. Psj cells were detected despite the surface sterilization. However, surface sterilization reduced the detection rate in barley. In the membrane filter method, Psj was detectable even in trace amounts after immersion of 100 g of wheat seed (2500 grains) for 4 days at 10°C in sterile distilled water, collecting the bacterial suspension on the membrane filter and applying it to selective medium. The method made it possible to detect Psj when only 0.08% of wheat seeds were contaminated.