Nanoparticle-Assisted Laser Desorption/Ionization Mass Spectrometry (Nano-PALDI MS) with Py-Tag for the Analysis of Small Molecules

Yukina Tatsuta; Yukie Tanaka; Akari Ikeda; Shigeru Matsukawa; Hajime Katano; Shu Taira

doi:10.5702/massspectrometry.S0069

Original Article

Nanoparticle-Assisted Laser Desorption/Ionization Mass Spectrometry (Nano-PALDI MS) with Py-Tag for the Analysis of Small Molecules

Yukina Tatsuta, Yukie Tanaka, Akari Ikeda, Shigeru Matsukawa, Hajime Katano, Shu Taira

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Keywords: proteomics, Py-Tag, LC-MS, stable isotope

JOURNAL OPEN ACCESS FULL-TEXT HTML

2017 Volume 6 Issue 3 Pages S0069

DOI https://doi.org/10.5702/massspectrometry.S0069

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Abstract

We compared two ionization methods, matrix assisted laser desorption/ionization (MALDI) and nanoparticle assisted laser desorption/ionization (Nano-PALDI) mass spectrometry (MS), for the analysis of amino acids derivatized with Py-Tag™ that consists pyrylium-based compound. Py-Tag is a useful stable derivatization reagent due to wide mass differences (using ¹³C as the sole stable labelling isotope). For Py-Tag labelled lysine, sensitive signals that showed less noise with a ten times higher sensitivity, showed a wider mass difference by Nano-PALDI MS compared to MALDI MS.

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