Microvascular Reviews and Communications
Online ISSN : 1880-5906
Print ISSN : 2188-1707
ISSN-L : 2188-1707
Cilostazol inhibits leukocyte-endothelial cell interactions in murine microvessels after transient bilateral common carotid artery occlusion
Takuya FukuokaTakeshi HayashiMakiko HirayamaHajime MaruyamaNorio Tanahashi
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2014 Volume 7 Issue 1 Pages 33

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Abstract

Leukocyte behavior in the cerebral microvasculature following vessel occlusion has not been fully elucidated. The purpose of this study was to investigate the effects of cilostazol on leukocyte behavior (rolling and adhesion) in murine cerebral microvessels following transient bilateral carotid artery occlusion using intravital fluorescence microscopy. Four groups of mice were assigned: a sham group (n=16); an ischemia (induced by 15-min occlusion of bilateral common carotid arteries) and reperfusion (I/R) group (n=13); I/R+cilostazol (I/R+CZ3mg/kg) group (I/R after oral administration of cilostazol at 3mg/kg) (n=8) and I/R+cilostazol (I/R+CZ30mg/kg) group (I/R after oral administration of cilostazol at 30mg/kg) (n=12). Leukocytes labeled with 0.05% acridine orange were administered intravenously and their behavior was investigated at 3 and 6 h after reperfusion. Numbers of rolling or adherent leukocytes were expressed as the count per square millimeter per 30s. Numbers of rolling and adherent leukocytes at 3 and 6h after reperfusion were significantly higher in the I/R group than in the sham or I/R+CZ30mg/kg groups in both pial veins (P<0.05) and pial arteries (P<0.05). Cilostazol (30mg/kg) inhibited leukocyte-endothelial interactions following cerebral ischemia and reperfusion.

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© 2014 by Japanese Society for Microcirculation
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