Functional genomics of the Dof transcription factor family genes in suspension-cultured cells of Arabidopsis thaliana

Yayoi Tsujimoto-Inui; Yuki Naito; Nozomu Sakurai; Hideyuki Suzuki; Ryosuke Sasaki; Hideyuki Takahashi; Namie Ohtsuki; Toshitsugu Nakano; Shuichi Yanagisawa; Daisuke Shibata; Hirofumi Uchimiya; Hideaki Shinshi; Kaoru Suzuki

doi:10.5511/plantbiotechnology.26.15

Original Paper

Functional genomics of the Dof transcription factor family genes in suspension-cultured cells of Arabidopsis thaliana

Yayoi Tsujimoto-Inui, Yuki Naito, Nozomu Sakurai, Hideyuki Suzuki, Ryosuke Sasaki, Hideyuki Takahashi, Namie Ohtsuki, Toshitsugu Nakano, Shuichi Yanagisawa, Daisuke Shibata, Hirofumi Uchimiya, Hideaki Shinshi, Kaoru Suzuki

Author information

Yayoi Tsujimoto-Inui
Institute for Biological Resources and Functions, AIST
Yuki Naito
Institute for Biological Resources and Functions, AIST
Nozomu Sakurai
Kazusa DNA Research Institute
Hideyuki Suzuki
Kazusa DNA Research Institute
Ryosuke Sasaki
Kazusa DNA Research Institute
Hideyuki Takahashi
Institute of Molecular and Cellular Biosciences (IMCB), The University of Tokyo
Namie Ohtsuki
Institute for Biological Resources and Functions, AIST
Toshitsugu Nakano
Institute for Biological Resources and Functions, AIST
Shuichi Yanagisawa
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo
Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST)
Daisuke Shibata
Kazusa DNA Research Institute
Hirofumi Uchimiya
Institute of Molecular and Cellular Biosciences (IMCB), The University of Tokyo
Hideaki Shinshi
Institute for Biological Resources and Functions, AIST
Kaoru Suzuki
Institute for Biological Resources and Functions, AIST

Keywords: Arabidopsis thaliana, cultured cells, Dof family, metabolic regulation, microarray, transcription factors, transgenic callus

JOURNAL FREE ACCESS

2009 Volume 26 Issue 1 Pages 15-28

DOI https://doi.org/10.5511/plantbiotechnology.26.15

Details

Abstract

The Dof (DNA-binding with one finger) genes are members of a family of plant-specific transcription factors that have a highly conserved DNA-binding domain, namely, Dof domain. The Dof domain is a particular class of zinc finger domain that has been demonstrated to bind specifically to DNA sequences with a T/AAAAG core. In the Arabidopsis genomic database, 36 Dof genes have been identified, whereas the functions of most of the members still remain to be studied. Therefore, we attempted to comprehensively and systematically investigate functions of Arabidopsis Dof genes. As the first step, we isolated cDNAs of all the Arabidopsis Dof genes based on the coding sequences identified on the genomic database. Then, we selected genes, which are subjected to further functional analysis, through a phylogenetic analysis, and transformed Arabidopsis cultured cells (line T87) using cDNAs corresponding to the selected genes. After that, we examined transcriptional profiles in the Dof gene-overexpressed calli using an Arabidopsis DNA microarray. From the results, possible involvements of the Dof genes in regulation of metabolic pathways are discussed.

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