Use of Liquefied Dimethyl Ether for the Extraction of Proteins from Vegetable Tissues

Hiroka FURUKAWA; Asuka KIKUCHI; Atsuko NORIYASU; François BOUTEAU; Syouhei NISHIHAMA; Kazuharu YOSHIZUKA; Xiaohong LI; Tomonori KAWANO

doi:10.15261/serdj.23.127

Notes

Use of Liquefied Dimethyl Ether for the Extraction of Proteins from Vegetable Tissues

Hiroka FURUKAWA, Asuka KIKUCHI, Atsuko NORIYASU, François BOUTEAU, Syouhei NISHIHAMA, Kazuharu YOSHIZUKA, Xiaohong LI, Tomonori KAWANO

Author information

Hiroka FURUKAWA
Faculty and Graduate School of Environmental Engineering, The University of Kitakyushu
Asuka KIKUCHI
Faculty and Graduate School of Environmental Engineering, The University of Kitakyushu
Atsuko NORIYASU
Faculty and Graduate School of Environmental Engineering, The University of Kitakyushu
François BOUTEAU
Université Paris Diderot, Sorbonne Paris Cité, Paris Interdisciplinary Energy Research Institute
LINV-DiSPAA, Department of Agri-Food and Environmental Science, University of Florence
University of Florence LINV Kitakyushu Research Center
Syouhei NISHIHAMA
Faculty and Graduate School of Environmental Engineering, The University of Kitakyushu
Kazuharu YOSHIZUKA
Faculty and Graduate School of Environmental Engineering, The University of Kitakyushu
Xiaohong LI
Faculty and Graduate School of Environmental Engineering, The University of Kitakyushu
Tomonori KAWANO
Faculty and Graduate School of Environmental Engineering, The University of Kitakyushu
University of Florence LINV Kitakyushu Research Center
Univ Paris Diderot, Sorbonne Paris Cité, Paris Interdisciplinary Energy Research Institute

JOURNAL FREE ACCESS

2016 Volume 23 Issue 1 Pages 127-135

DOI https://doi.org/10.15261/serdj.23.127

Details

Abstract

Dimethyl ether (DME), the simplest ether with the formula CH₃OCH₃, is a low-temperature solvent and extraction agent applicable to specialized laboratory procedures as recently demonstrated for extraction of biologically active, flavoring or pungent organic compounds from some biological materials. Due to its low boiling point, DME facilitates the removal of solvent from the samples after extraction. In the present study, we demonstrated the extraction of proteins from juicy or relatively dry vegetable tissues and the distribution of proteins in the dry phase and separated aqueous phase were compared. It is notable that a series of proteins from carrot roots, sized between 84.7 and 33.1 kDa, were detected in the liquid sample extracted by DME, suggesting that DME could be used as an effective extraction solvent for separating the hydrophilic (water soluble) proteins from the crude protein samples. Extraction of water-soluble proteins largely depends on the de-watering action of DME.

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