The subject of evolution has been newly introduced to high school biology classes in Cambodia as part of a new national curriculum. This study reports on the development of teaching materials for teaching evolution in high school biology classes in Cambodia. These developed teaching materials utilize a duck embryo, which is commonly eaten in Cambodia as pong tia koon. The effectiveness of the developed teaching materials was examined by distributing the materials to Cambodian university students. Our main results are as follows: (1) We can observe the embryonic duck’s development using an incubator; (2) We developed new teaching materials for teaching evolution in Cambodian biology classes; (3) Students who use these teaching materials think that they are interesting and helpful for understanding evolution. Thus, we conclude that the developed teaching materials are very effective for teaching evolution in Cambodia.