Conducting Polymer Based Nucleic Acid Sensor for Environment Monitoring

Bansi Dhar MALHOTRA; Nirmal PRABHAKAR; Pratima R. SOLANKI

doi:10.1093/ietele/e91-c.12.1889

Special Section on The Forefront of 21st Century Organic Molecular Electronics

Conducting Polymer Based Nucleic Acid Sensor for Environment Monitoring

Bansi Dhar MALHOTRA, Nirmal PRABHAKAR, Pratima R. SOLANKI

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Keywords: nucleic acid sensor, electrochemical, polyaniline, perchlorate, arsenic trioxide

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2008 Volume E91.C Issue 12 Pages 1889-1893

DOI https://doi.org/10.1093/ietele/e91-c.12.1889

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Abstract

Nucleic acid sensor based on polyaniline has been fabricated by covalently immobilizing double stranded calf thymus (dsCT) DNA onto perchlorate (ClO^-₄) doped polyaniline (PANI) film deposited onto indium-tin-oxide (ITO) glass plate using 1-(3-(dimethylamino) propyl)-3-ethylcarbodiimide hydrochloride (EDC)/N-hydroxyl succinimide (NHS) chemistry. These dsCT-DNA-PANI/ITO and PANI/ITO electrodes have been characterized using square wave voltammetry, electrochemical impedance, and Fourier-transform-infra-red (FTIR) measurements. This disposable dsCT-DNA-PANI/ITO bioelectrode is stable for about four months, can be used to detect arsenic trioxide (0.1ppm) in 30s.

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