2000 Volume 28 Issue 1 Pages 13-17
The present study attempts to determine standard banding patterns for Group specific component (GC), Hemoglobin (HB), Plasminogen (PLG) and Transferrin (TF) of Elk and Japanese sika deer by polyacrylamide gel isoelectric focusing (IEF) - immunoblotting technique and horizontal alkaline polyacrylamide gel electrophoresis (PAGE) . Blood samples of 13 Elk were sent to us from Canada blood center for International Comparison Test in 1995, and 56 Japanese sika deer in three areas Hokkaido, Kinkazan and Tsushima, sent to us from Japanese Association of Deer Farming during 1996 to 1998 were used. TF banding patterns showed the same type in both ELK and Japanese sika deer, while the banding patterns of other proteins were different in both specifies. Polymorphism was found in GC of ELK and TF of Japanese sika deer. In addition, polymorphism for TF was found in deer populations from Hokkaido and Tsushima of three areas, amd its allele frequency differed significantly among three populations (P<0.01) .