The Journal of Animal Genetics
Online ISSN : 1884-3883
Print ISSN : 1345-9961
ISSN-L : 1345-9961
Comparison of genetic variation of group-specific component, hemoglobin, plasminogen and transferrin between Elk (Cervus canadensis) and Japanese sika deer (Cervus nippon)
Emiko FukuiSusumu MuramatsuMidori Yoshizawa
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Keywords: deer, polymorphism, protein
JOURNAL FREE ACCESS

2000 Volume 28 Issue 1 Pages 13-17

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Abstract

The present study attempts to determine standard banding patterns for Group specific component (GC), Hemoglobin (HB), Plasminogen (PLG) and Transferrin (TF) of Elk and Japanese sika deer by polyacrylamide gel isoelectric focusing (IEF) - immunoblotting technique and horizontal alkaline polyacrylamide gel electrophoresis (PAGE) . Blood samples of 13 Elk were sent to us from Canada blood center for International Comparison Test in 1995, and 56 Japanese sika deer in three areas Hokkaido, Kinkazan and Tsushima, sent to us from Japanese Association of Deer Farming during 1996 to 1998 were used. TF banding patterns showed the same type in both ELK and Japanese sika deer, while the banding patterns of other proteins were different in both specifies. Polymorphism was found in GC of ELK and TF of Japanese sika deer. In addition, polymorphism for TF was found in deer populations from Hokkaido and Tsushima of three areas, amd its allele frequency differed significantly among three populations (P<0.01) .

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© Japanese Society of Animal Breeding and Genetics
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