Polymorphism in the acetylcholinesterase gene of the housefly, Musca domestica L. (Diptera: Muscidae)

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The cDNA fragments of a 2 kb gene, encoding the housefly acetylcholinesterase (AChE) were compared among 6 strains. While there were 4 substitutions of amino acids in the predicted peptides between Asp₈₃ and Arg₆₄₈, which is well conserved in insects, the following contrasts were made among the strains. The insecticide susceptible strains, aabys and SRS had Gly₃₄₂ and Phe₄₀₇, and the resistant strains, LPR, YBOL and YPRN had Ala₃₄₂ and Tyr₄₀₇, with Val₃₄₂ unique to YBOL. In addition, Leu₂₆₀ was found only in the chiral organophosphate resistant strains, YBOL and YPRN. These results showed that the patterns of single nucleotide polymorphisms, which resulted in amino acid substitutions, can be a practical tool for characterization of the insecticide resistance of houseflies.