Online ISSN : 1347-5800
Print ISSN : 0044-5991
ISSN-L : 0044-5991
Protective Effect of Ebselen on Ischemia-reperfusion Injury in Epigastric Skin Flaps in Rats
Takahiko KurokiSusumu TakekoshiKanae KitataniChikara KatoMuneo MiyasakaTadashi Akamatsu
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Supplementary material

2022 Volume 55 Issue 5 Pages 149-157


The purpose of this study was to determine the role of oxidized diacylglycerol (DAG) and the molecular mechanism underlying ischemia-reperfusion (I/R) injury in rat skin flaps. The protective effect of ebselen on the viability of rat skin flaps with I/R injury was investigated. Flaps were designed and raised in the left inguinal region. Then, a microvascular clamp was applied to the vascular pedicle and reperfused after 6 hr. After 7 days of I/R (I/R group), the skin flap survival area ratio was significantly reduced compared to the normal skin. The administration of ebselen significantly improved the ratio compared to the I/R group. The flap survival area ratio of the I/R + ebselen group was significantly improved compared to the I/R + vehicle group. In the I/R + ebselen group, the oxidized DAG content and intensity of phosphorylated PKCα and PKCδ were significantly lower compared to the I/R + vehicle group. Furthermore, the inflammatory response was suppressed in the I/R + ebselen group compared to the I/R + vehicle group. These results indicate that ebselen is useful as a preventive and therapeutic agent for skin flap necrosis caused by I/R, because of reduction and elimination of oxidized DAG.

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© 2022 The Japan Society of Histochemistry and Cytochemistry

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