METHYLENE BLUE-AZURE B-EOSIN STAINING METHOD FOR THIN METHACRYLATE SECTIONS OF BONE MARROW

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Bone marrow specimens were removed from the femurs of gerbils perfused with 10% buffered formalin. They were embedded in methacrylate and sectioned on a Sorvall JB-4 microtome at 1 to 3μm at 0.5μm intervals. Staining was accomplished with methylene blue-azure B-eosin as follows: Slides were immersed in the methylene blue-azure B mixture for 1min, rinsed in 95% ethyl alcohol, immersed in ethanol eosin, decolorized in acid alcohol, and dehydrated in 100% methyl alcohol, acetone and xylene. The entire staining procedure takes about 10min and yields results comparable to those derived from Wright-Giemsa stained film preparations. It allows easier and more reliable identification of the various members of the myeloid and normoblastic cell lines than hematoxylin and eosin stained bone marrow sections.