IMMUNOHISTOLOGICAL STUDY OF EQUINE RENAL METALLOTHIONEIN
1978 年 11 巻 2 号 p. 205-211
詳細
1978 年 11 巻 2 号 p. 205-211
The cellular site of metallothionein, a cadmium-and zinc-binding protein, was investigated by means of immunofluorescent antibody technique in several organs of horse, human, monkey, pig, bovine, mouse and rat. The antisera to the metallothionein was raised in rabbits. A single precipitin band was observed between antisera and antigen using both agar diffusion method and immunoelectrophoresis.
The material reacting with anti-equine metallothionein antisera has been detected in the renal tissue of horse, human and monkey, but not of pig, bovine, mouse and rat. Metallothionein was mainly found in the cytoplasms of proximal convoluted tubules and Henle loop of these kidneys. No remark-kable positive fluorescence was found in other organs. The concentration of cadmium in the kidneys of these animals was measured as 30-130ppm in horse and monkey, and 0.1-0.3ppm in pig, cow, mouse and rat.
