A MODIFIED ALUMINUM-FORMALDEHYDE (ALFA) HISTOFLUORESCENCE METHOD FOR ENHANCED VISUALIZATION OF CENTRAL CATECHOLAMINE

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We designed a modification of the aluminum-formaldehyde (ALFA) histofluorescence method based on preperfusion with buffer containing formaldehyde (FA). This modified ALFA technique enables visualization of all catecholamine (CA) neuron systems with a sensitivity comparable to that of the previously published glyoxlic acid (GA)-Vibratome method, providing better reproducibility and facility of the procedure than the ALFA method.