A Simplified Method of Staining Vitamin A Lipid Droplets in Stellate Cells at the Light and Electron Microscopic Levels
1996 年 29 巻 2 号 p. 147-151
詳細
1996 年 29 巻 2 号 p. 147-151
A gold chloride method for demonstration of retinyl esters (vitamin A) -storing stellate cells (Ito cells) in liver by Wake et al (Stain Technol. 61: 193, 1986) was modified. The procedure utilizes a microslicer instead of a freezing microtome. With the use of this procedure, the reaction product was fine and stable. Sections of the same tissue-block can be observed at the light, fluorescence and electron microscopic leveles simultaneously. The black reaction product produced between retinyl esters and gold chloride is localized in the vitamin A lipid droplets. Using a low concentration of gold chloride staining solution (0.001%-0.0001%), non-specific precipitate was significantly reduced at the electron microscopic level.
