ACTA HISTOCHEMICA ET CYTOCHEMICA
Online ISSN : 1347-5800
Print ISSN : 0044-5991
ISSN-L : 0044-5991
AN ACRIFLAVINE ALCIAN BLUE TECHNIQUE FOR DUAL STAINING OF CARTILAGE AND MAST CELLS IN PARAFFIN SECTIONS
KAZUYORI YAMADA
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1970 年 3 巻 1 号 p. 1-6

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Dewaxed and hydrated sections were treated with a 0.5% acriflavine hydrochloric acid solution of pH 1.5 for 30 min and then washed with distilled water followed by a rinse in descending concentration of ethanol and again in water. The sections were next treated with a 0.1% alcian blue acetic acid solution of pH 2.5 for 2-20 min, rinsed in water, dehydrated with ethanol and then cleared and mounted. Cartilage matrix appears bluish yellow and lacunar borders and chondrocyte cytoplasm yellowish blue. Mast cell cytoplasmic constituents appear bluish orange in contrast to the pale yellow of the nucleus.

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© the Japan Society of Histochemistry and Cytochemistry
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