HISTOCHEMICAL DEMONSTRATION OF THE ACTIVITY OF RIBOSE-5-PHOSPHATE ISOMERASE IN HUMAN PLACENTA AND BOVINE HEART MUSCLE
1975 年 8 巻 2 号 p. 134-138
詳細
1975 年 8 巻 2 号 p. 134-138
Ribose-5-phosphate isomerase was histochemically demonstrated in cryostat sections of the human placenta and the bovine heart muscle containing a conduction system. Suitable conditions for histochemical reaction of enzyme activity in those tissues were determined. The incubation mixture containing 50mg ribose-5-phosphate, 10mg NADP, 10ml Nitro-BT (1mg/ml), 10ml propandiol-HCl buffer (0.2M pH 8.0), 0.04ml 6-phosphogluconate dehydrogenase solution (1mg/ml), 2ml 3% gelatine solution and 2mg MnCl2 was proposed, while two control procedures were prepared, using the incubation mixture without ribose-5-phosphate and without 6-phosphogluconate dehydrogenase.
