This study aims to clarify the building regulations in agricultural district of Yamashiro province through the Edo period. This paper takes up the building regulations by Kyoto machibugyosho from the 17^<th> to the 18^<th> century, and compares them with the building regulations by the Tokugawa Shogunate. As the result of that, the building development and the new housing building are prohibited in agricultural district of Yamashiro province by Kyoto machibugyosho, which afe similar to the building regulations by the Tokugawa Shogunate. But, in agricultural district of Yamashiro province, the building regulations had changed in the first place result from the flood and others.