The purpose of this research is to analyze MVOC emissions from microorganisms in HVAC systems and to survey their effect on indoor air quality. MVOC emissions from fungi sampled in HVAC systems were measured in the laboratory. 2-methyl-2-Propanol, 2-Pentanone, methyl isobutyl Ketone and others MVOC were emitted from Cladosporium, Aspergillus, Penicillium and Alternaria. MVOC emissions from each fungus were different according to the fungi genus though some MVOC were emitted from all fungus. And it was found from the field measurement that MVOC thought to be from fungi emissions such as 2-methyl-1-Propanol, 2-Pentanone and methyl isobutyl Ketone, were emitted by some HVAC systems to indoor. For these results, it is suggested that MVOC emissions from fungi in HVAC system became one of the indoor pollution sources.