The purpose of this study is to compare and analyze the attributes of male and female subjects (evacuation supporters), transportation preparation time according to the size of transportation preparation space, and vertical transportation efficiency of stair ascent. For this, this study reenacted transportation situations of vulnerable people using stretchers and measured the transportation preparation time, the transportation speed and vertical transport efficiency of stair ascent transportation. As a result, in case of minimum operating space for transportation preparation, it was found that there was more than one minute of waiting time to start evacuation support actions. In addition, the transportation amount was around 0.02 ~ 0.25 persons/sec, and the transportation amount of the condition 2(condition of starting in a queue) that started transportation in a straight line was higher than the condition 1(condition of starting from scattered points). And the transportation amount of the female transportation team was relatively lower than the male transportation groups.