Simultaneous Catalyzing of Uricase- and Catalase-Like Reactions of Resins Modified with Metalloporphyrins and Its Application for Determination of Uric Acid¹

Abstract

Some anion-exchange resins modified with water-soluble metalloporphyrins exhibited an ability to catalyze simultane-ously both uricase- and catalase-like reactions. The resin modified with manganese-tetrakis(4-sulfophenyl)porphine (Mn-TPPS_r), which showed the highest activity among the resins tested, was useful for the indirect determination of uric acid by measuring the amount of formaldehyde produced. The calibration curve was linear over the range from 10 μg to 200 μg of uric acid with a relative standard deviation of 1.8% (n=10) for 200 μg uric acid. Mn-TPPS_r was also applied for the determination of uric acid in sera.