1999 Volume 15 Issue 11 Pages 1059-1064
Secondary equilibrium chromatography has proved a useful tool for determining the polyether complexation constants. Both a cation-exchange and anion-exchange method can be used for this purpose. In the present paper, the applicability and versatility of the latter method, based on the surface complexation of polyethers with ammonium ions anchored on the stationary phase surface, are discussed, and compared with those of the former established method. The precision of the chromatographically determined complexation constants depends on the range of measured retention factors, suggesting that the surface charge density of the stationary phase can be optimized according to the retention ability of polyethers. The anion-exchange method is more versatile than the cation-exchange method from this viewpoint; i.e. the surface density of active groups (thus the retention range) can simply be controlled by preequilibrating the stationary phase with an aqueous solution of appropriate pH.