Flow-Injection Determination of Vitamin K₃ by a Chemiluminescence Sensor

Abstract

A chemiluminescence (CL) sensor for vitamin K₃ (menadione sodium bisulfite, MSB) combined with a flow-injection system is presented in this paper. It is based on the auto-oxidation of bisulfite liberated from MSB in alkaline media in the presence of Tween 80 sensitized by Rhodamine 6G immobilized on a cation-exchange column. The sensor responds linearly to the MSB concentration in the range of 0.5 - 10 μg/ml with a detection limit (3σ) of 2.6 μg/l. The analysis can be performed within 1 min with a relative standard deviation of <5%. The sensor is stable for over 250 determinations and has been successfully applied to the determination of MSB in injections and tablets.