Analytical Sciences
Online ISSN : 1348-2246
Print ISSN : 0910-6340
ISSN-L : 0910-6340
Original Papers
Fluorometric Determination of Glucose by Flow Injection Analysis Using Immobilized Enzyme-Reactor Columns Prepared by Coupling Glucose Oxidase and Peroxidase onto Chitosan Beads
Tetsuyuki TANIAIAkio SAKURAGAWATadao OKUTANI
Author information
JOURNALS FREE ACCESS

2000 Volume 16 Issue 5 Pages 517-521

Details
Abstract

A combination technique of some kinds of enzymatic reactions for flow injection analysis with an immobilized enzyme-reactorcolumn was studied. This system consisted of hand-made reactor columns packed with glucose oxidase and horseradish peroxidase immobilized onto chitosan beads, and a fluorometric detector. It was applied for the determination of glucose in some beverages, Japanese Sake and liquor samples. Under the recommended conditions, it could determinea sample within 10 min. The calibration curve for glucose was linear from 20.0 mg dm-3 to 0.5 mg dm-3 in the low-sensitivityregion of the detector, and from 0.5 mg dm-3 to 30 g dm-3 in the high-sensitivity region of the detector. The detection limit (S/N=10) was 30 µg dm-3 as the concentration of glucose, and 54 ng as its absolute amount. There was no interference from major substances present in either beverage or serum samples.

Information related to the author
© 2000 by The Japan Society for Analytical Chemistry
Previous article Next article
feedback
Top