Bulk Acoustic Wave Sensing Technique Applied to Dibazol Assay in Serum and Urine

Abstract

A simple, rapid, specific and sensitive method for the assay of dibazol using a bulk acoustic wave technique is described. PVC film containing various activants was coated on one electrode of a piezoelectric quartz crystal to fabricate a dibazol bulk acoustic wave (BAW) sensor for the assay of dibazol. Neither a sample pretreatment nor separation was required. The logarithm of the frequency shift of the dibazol BAW sensor shows a linear relationship to the logarithm of the concentration of dibazol over the range 4.0 × 10^-8 - 9.0 × 10^-4 M with a detection limit of 4.0 × 10^-8 M. In this work, three kinds of ion-pair complexes, namely, dibazol with dicyclohexylnaphthalene sulfonate (DDCHNS), diisobutylnaphthalene sulfonate (DDBNS) and tetraphenylborate (DTPB), were synthesized and investigated. The influencing factors were also examined and optimized. A comparison of the BAW sensing technique with other methods for assaying dibazol was made.