2001 Volume 17 Issue 12 Pages 1425-1429
An iron(III) complex of thiacalixarenetetrasulfonate on a modified anion-exchanger (Fe3+-TCASA-500) has shown high peroxidase-like activity at pH 5 - 6 for the reaction of quinoid-dye formation between 3-methyl-2-benzothiazolinone hydrazone and N-(3-sulfopropyl)aniline in the presence of hydrogen peroxide. Utilizing the peroxidase-like activity of Fe3+-TCASA-500 for this reaction, a method using Fe3+-TCASA-500 was applied for the spectrophotometric determination of hydrogen peroxide. The calibration curve by the method using Fe3+-TCASA-500 was linear over the range from 1 to 10 μg of hydrogen peroxide in a 1 ml sample solution. The apparent molar absorptivity for hydrogen peroxide was 2.4 × 104 l mol-1 cm-1, which was about 80% of that by peroxidase under the same conditions. This determination method of hydrogen peroxide using Fe3+-TCASA-500 was applied for the determination of glucose in diluted normal and abnormal control serum I and II.